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Oxidative stress reduces glycolytic flux by oxidation of the active site cysteine of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase, which inactivates the enzyme within seconds of oxidative stress, or in response to endogeneous increases in oxidant levels, for instance, upon anchorage-independent growth of cancer cells 23,34–37

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2 Materials and methods 2.1 Bacterial strains and reagents E